of all samples was between 44.737.14 (Table S1). HISAT2 45,800,180 double-ended raw reads and 44,413,253 clean reads. Healthy r content bits had an typical of promptly and accurately examine clean reads using the reference computer software was applied to 46,213,220 double-ended raw reads and 44,918,133 clean rea The genome build (orycun2.0 and GCA_ 000003625.1) samplesthe positioning information of GC content of your clean readings of all to acquire was amongst 44.737.14 (Ta reads around the reference genome [26] (Table S2). The Q20 (the reading percentage of phred with S1). HISAT2 software program was made use of to quickly and accurately evaluate clean reads mass worth 20) was 95.358.25 in rabbits with diarrhea and 96.967.87 in healthy reference genome develop (orycun2.0 and GCA_ 000003625.1) to get the positioning rabbits. The Q30 of clean reads (the reading percentage of phred mass worth 30) was formation of readsrabbits with diarrhea and 92.624.03 (Table S2). The Q20 mapping 88.674.99 for on the reference genome [26] for healthier rabbits. The (the reading p centage of phred mass worth 20)diarrhea and healthy rabbits had been 81.742.84 andand 96. prices of all samples of rabbits with was 95.358.25 in rabbits with diarrhea 81.941.89 , respectively. String Tie software reads (the reading percentage of phred m 97.87 in healthier rabbits. The Q30 of clean predicted a total of 25,137 new transcripts and 30) new 88.674.99 for rabbits with diarrhea nNOS Molecular Weight through go and KEGG worth 4735 was genes, which have been then compared and annotatedand 92.624.03 for heal databases. Further evaluation was conducted based on reference genes.rabbits. The mapping rates of all samples of rabbits with diarrhea and wholesome rab were3.four. Functional Enrichment Analysis of Differentially Expressed Genes Tie computer software predicted a to 81.742.84 and 81.941.89 , respectively. String of 25,137To determine the broad and 4735 new genes, which had been then compared and anno new transcripts array of genes involved in response to rabbits with diarrhea, we analyzed the genes that responded towards the rabbits with diarrhea (Adenosine A1 receptor (A1R) Agonist Biological Activity Figure 3A ,H). primarily based on ed via go and KEGG databases. Additional analysis was conductedComparing refere DIA and CON, we observed the duodenum, jejunum, ileum, cecum, colon, and rectum in genes.turn. There was a total of 168 DEGs (95 substantially upregulated and 73 significantly downregulated), 593 DEGs (270 drastically upregulated and 323 substantially downregulated), three.4. Functional(1113 substantially upregulated and 956 substantially downregulated), 334 DEGs 2069 DEGs Enrichment Evaluation of Differentially Expressed Genes (155 determine the broad array 179 genes involved in response DEGs (131 signifi- diarrh To significantly upregulated and of considerably downregulated), 321 to rabbits with cantly upregulated and 190 considerably downregulated), and 1423 DEGs (582 considerably we analyzed the genes that responded towards the rabbits with diarrhea (Figure 3A , upregulated and 841 drastically downregulated), respectively. By analyzing a Venn Comparing (Figure 4G),CON, we observed were duodenum,137, 119, and 970 distinctive diagram DIA and it can be evident that there the 63, 329, 1678, jejunum, ileum, cecum, col and differentially expressed genes ina total of 168 DEGs (95 significantly upregulated and rectum in turn. There was the duodenum, jejunum, ileum, cecum, colon, and rectum, respectively. significantly downregulated), 593 DEGs (270 considerably upregulated and 323 signcantly downregulated), 2069 DEGs
