EJin et al. BMC Genomics(2022) 23:Web page eight ofFig. 3 Twelve candidate genes had been
EJin et al. BMC Genomics(2022) 23:Page 8 ofFig. three Twelve candidate genes have been verified and measured by real-time fluorescence quantitative PCR. The data represent the Thrombopoietin Receptor Source typical typical deviation (n = three). The vertical axis represents the level of gene expression discovered by means of PCR. There was a substantial difference in between tea leaves treated with brassinosteroids (BRs) for 0 h and tea leaves exogenously sprayed with BRs for 48 h (P 0.05)also identified (Fig. four: two). KEGG evaluation showed that compared with CAK (BR spraying for 0 h), the expression levels of many mitotic cyclin genes which include Cyc, CycD3, CycD4, and CDC6 have been upregulated three h, 9 h, 24 h, and 48 h after BR spraying, but the highest gene expression levels varied among time points. We hypothesize thatwithin 48 h of BR spraying, cyclin genes have been upregulated, which in turn promoted development through cell division. Furthermore, it was found that spraying BRs onto tea leaves also substantially upregulated the cold resistance genes CBF and ICE, as well because the theanine synthesis-related genes threonine synthase, (TS), glutamineJin et al. BMC Genomics(2022) 23:Web page 9 ofFig. 4 1 Heat map of genes related to BR signal transduction. 2 Heat map of genes associated with cell Bacterial supplier division, theanine, caffeine, and cold resistance. three Chlorophyll synthesis pathway; heat map of genes associated with chlorophyll synthesis. four Starch synthesis pathway; heat map of genes associated with starch synthesis. 5 Sucrose biosynthesis pathway; heat map of genes related to sucrose synthesis. 6 Flavonoid biosynthesis pathway; heat map of genes related to flavonoid biosynthesis. Red and green represent higher expression levels and low expression levels, respectively; CAK, Brassinosteroids (BRs) sprayed for 0 h; CAA, BRs sprayed for 3 h; CAB, BRs sprayed for 9 h, CAC; BRs sprayed for 24 h; CAD, BRs sprayed for 48 hsynthetase (GS), and arginine decarboxylase (ADC). Interestingly, the expression of caffeine-related synthetic genes was downregulated for example caffeine synthase two(TCS2) and S-adenosylmethionine synthase (SAMS).Exogenous spraying of BR upregulates genes associated with the chlorophyll biosynthetic pathway in tea leavesKEGG enrichment annotation identified 5 genes inside the ginseng chlorophyll biosynthesis pathway (Fig. four: 3). KEGG evaluation showed that compared with CAK (BR spraying for 0 h), immediately after BR spraying for three h, 9 h, 24 h, and 48 h, the essential regulatory genes of glutamate-1-semialdehyde aminotransferase (GSA), uroporphyrinogen III synthase (HEMD), POR, Mg-chelatase (C-HLH), and chlorophyllide a oxygenase (CAO) which might be associated with chlorophyll synthesis pathway have been upregulated, and their expression levels peaked at 48 h.Exogenous spraying of BR onto tea leaves promotes the upregulated expression of genes related to the starch biosynthesis pathwayKEGG enrichment annotation revealed that 3 genes are involved inside the starch biosynthesis pathway (Fig. 4: 4). KEGG evaluation showed that compared with CAK (spraying BRs for 0 h), after spraying BRs for 3 h, 9 h, 24 h, and 48 h, the expression of ADP-Glc pyrophosphorylase (AGPase), GBSS, phosphoglucomutase (PGM), along with the starch-branching enzyme (SBE) important regulatory bases associated with the starch synthesis pathway had been upregulated. At 24 h, the expression of genes related to the sucrose synthesis pathway peaked.Exogenous spraying of BR onto tea leaves promotes the upregulated expression of genes inside the sucrose biosynthetic pathwayEight genes involved inside the sucrose biosynthesis path.
