(1113 drastically mGluR2 drug upregulated and 956 significan downregulated), 334 DEGs (155 substantially upregulated and 179 significantly dow regulated), 321 DEGs (131 substantially upregulated and 190 considerably downreguAnimals 2021, 11, x FOR PEER REVIEW6 ofAnimals 2021, 11,ed), and 1423 DEGs (582 significantly upregulated and 841 substantially downregulated), respectively. By analyzing a Venn diagram (Figure 4G), it can be evident that there were 63, 329, 1678, 137, 119, and 970 exceptional differentially expressed genes inside the duodenum, jejunum, ileum, cecum, colon, and rectum, respectively.6 ofA.B.C.D.E.F.G.H.Figure 3. Differential gene volcano map, differential gene Wayne map, and differential gene quantity histogram. (A ) The Vocalo diagram evaluation of DEGs inside the duodenum, jejunum, ileum, cecum, colon, and rectum in turn. Red indicates considerably upregulated DEG, and blue indicates drastically downregulated DEG (|log2(foldchange)| 1 and p-value 0.05). (G) Differential gene Venn diagram; distinct colors indicate distinct comparison combinations. (H) The histogram of your quantity of differentially expressed genes is shown in blue and gray. The quantity around the column represents the amount of differentially expressed genes. S_Z: the duodenum of healthy rabbits, S_B: diarrhea inside the duodenum of rabbits, H_Z: healthier rabbit ileum, H_B: diarrheal rabbit ileum, K_Z: healthier rabbit jejunum, K_B: rabbits with diarrheal jejunum, M_Z: healthy cecum of rabbits, M_B: rabbits with diarrheal cecum, J_Z: healthy rabbit colon, J_B: colon of rabbits with diarrhea, Z_Z: healthful rabbit rectum, Z_B: rectum of rabbits with diarrhea.36.Yunpeng, C.; Shihua, P.; Jianjun, Z.; Weijie, Z.; Huijuan, N.; Zhicai, Q.; Jingwen, W.; Daleng, S.; Zengji, Y. RAPD Evaluation of Genetic Relationships among Natural Populations of Hybrid Taxodium Mucronatum Tenore. Fu Dan Xue Bao Zi Ran Ke Xue Ban J. Fudan Univ. Nat. Sci. 2002, 41, 64145.Animals 2021, 11,7 ofBiosensors 2021, 11, x FOR PEER REVIEW13 μ Opioid Receptor/MOR web ofFigure 4. Cont.Animals 2021, 11,8 ofFigure four. GO enrichment analysis of DEGs in distinct comparison groups. S_Z vs. S_B (A), K_Z vs. K_B (B), H_Z vs. H_B (C), M_Z vs. M_B (D), J_Z vs. J_B (E), and Z_Z vs. Z_B (F). GO terms are on the x-axis. The enrichment ratio of genes is shown as GO terms for BP, CC, and MF. S_Z: the duodenum of wholesome rabbits, S_B: diarrhea inside the duodenum of rabbits, H_Z: healthful rabbit ileum, H_B: rabbit with diarrheal ileum, K_Z: wholesome rabbit jejunum, K_B: rabbits with diarrheal jejunum, M_Z: wholesome cecum of rabbits, M_B: rabbits with diarrheal cecum, J_Z: healthier rabbit colon, J_B: colon of rabbits with diarrhea, Z_Z: wholesome rabbit rectum, Z_B: rectum of rabbits with diarrhea.3.five. Enrichment Analysis of GO and KEGG Pathway Compared with the general description from the properties of genes or transcripts with functional annotation, the lowest level of gene function and KEGG pathway may be annotated by enrichment analyses. Moreover, enrichment evaluation delivers essentially the most detailed information regarding gene function and KEGG pathway, which can assist us to screen unique insights on diarrhea response in rabbits fed a no-antibiotic diet. Furthermore, GO terms and KEGG pathways that satisfy the corrected p-value of 0.05 were regarded drastically enriched. To classify and characterize DEG functions and pathways, we performed a gene ontology (GO) classification and functional annotation of molecular biological function, cellular elements, and biological procedure (F
