ntributions NM, GJD, HSO, and JGB created and planned the research. MH ready fungal cultures. CB and SS ready activitybased probes utilised within this study. NM collected CD30 site secretome samples and performed activitybased protein profil ing experiments. NM collected and analysed proteomic information. DN performed bioinformatic analysis. NM and MS ready P. pastoris strains, developed and purified recombinant enzymes, and performed activity assays. NM wrote the manuscript with input from all the authors. All authors read and approved the final manuscript. Funding The authors thank the All-natural Sciences and Engineering Investigation Council of Canada (PostDoctoral Fellowship to NGSM), the Royal Society (Ken Murray Research Professorship to GJD), the Biotechnology and Biological Sciences Research Council (BBSRC) (grant BB/R001162/1 to GJD), the French National Research Agency (ANR13BIME0002 to JGB), the Netherlands Organization for Scientific Analysis (NWO Prime grant 2018714.018.002 to HSO), and the European Research Council (ERC2011AdG290836 “Chembiosphing” to HSO, ERC2020SyG951231 “Carbocentre” to GJD and HSO). Proteomics information have been collected at the York Centre of Excellence in Mass Spectrometry, which was developed thanks to a significant capital investment by way of Science City York, sup ported by Yorkshire Forward with funds in the Northern Way Initiative, and subsequent help from EPSRC (EP/K039660/1; EP/M028127/1). Availability of information and supplies Pichia pastoris strains and samples of recombinant proteins may well be readily available from Gideon Davies ([email protected]). Samples of ABPCel, ABPXyl, and ABPGlc may be offered from Herman Overkleeft (h.s.overkleeft@lic. leidenuniv.nl). Basidiomycete fungi are offered in the fungal culture collection with the International Centre of Microbial Sources (CIRMCF) in the French National Institute for Agricultural research (INRA; Marseille, France). Genome sequences for each and every from the fungi utilized within this study are readily available from Mycocosm (mycocosm.jgi.doe.gov/mycocosm/home) (DOE Joint Genome Institute, Walnut Creek, California). Other Estrogen receptor list datasets used and/or ana lysed through the existing study are out there from the corresponding author on affordable request.Author facts 1 York Structural Biology Laboratory, Division of Chemistry, The University of York, Heslington YO10 5DD, York, UK. two Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands. 3 UMR1163 Bio diversitet Biotechnologie Fongiques, Facultdes Sciences de Luminy, INRAE, Aix Marseille Univ, 13288 Marseille, France. 4 Polytech Marseille, Aix Marseille Univ, 13288 Marseille, France. Received: eight October 2021 Accepted: 6 JanuaryDeclarationsEthics approval and consent to participate Not applicable. Consent for publication Not applicable. Competing interests The authors declare no competing interests.References 1. Scheller HV, Ulvskov P. Hemicelluloses. Annu Rev Plant Biol. 2010;2(61):2639. 2. Luis AS, Briggs J, Zhang X, Farnell B, Ndeh D, Labourel A, et al. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018;three(two):210. 3. Celiska E, Nicaud JM, Bialas W. Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide sources: critique on starch, cellulose, xylan, and inulin. Appl Microbiol Biotechnol. 2021;105(three):9759. 4. Schlembach I, Hosseinpour Tehrani H, Blank LM, B hs J, Wierckx N, Regestein L, et al. Consolidate
