Ure 1B). Ultimately, a total of 15 coexpression modules were constructed (Figure 1C). A total of 860 genes, accounting for 16.62 , had been not assigned to any of those modules. We assigned a color to and counted the amount of genes in each module. There have been 863 genes in the turquoise module, 788 within the blue module, 635 in the brown module, 432 in the yellow module, 382 in the green module, 270 within the red module, 205 in the black module, 192 within the pink module, 179 inFrontiers in Veterinary Science | www.frontiersin.orgJuly 2021 | Volume 8 | ArticleLiu et al.Network for E. tenella Infected Chickenthe magenta module, 134 within the purple module, 76 within the greenyellow module, 47 within the tan module, 42 inside the salmon module, 37 in the cyan module, and 33 within the midnight blue module.Infection Status Connected Modules AnalysisTwo modules of yellow and magenta had been substantially TXB2 Molecular Weight associated with the infection status (principal vs. secondary infection) by the module-trait evaluation (Figure 1E). For module yellow, genes were involved in biosynthetic and metabolism method (nucleoside phosphate, organophosphate, and carbohydrate derivative) (Figure 3A) and metabolism pathways (histidine, tyrosine, drug metabolism-cytochrome P450, tryptophan and phenylalanine) (Figure 3B). The expression amount of Genes in yellow module decreased drastically inside the main infection over time and elevated in the secondary infection over time (Figure 3C). The concept networks showed the details of genes in the prime 5 GO terms and KEGG pathways (Figures 3D,E). The coexpression network for module yellow genes was shown in Figure 3F. Genes in module magenta were involved in immune response, defense response and actin filaments associated functions (Figure 4A). The expression level of genes in this module increased substantially in the principal infection more than time (Figure 4B). The idea networks showed the details of genes within the best 11 GO terms and IRF1, IFNG, and CAPZA1 were circled as the significant genes (Figure 4C) and also identified as hub genes in this module (Figure 4D).Module Stability TestThe module stability showed that module pink, turquoise, blue, purple, green and black were among one of the most steady modules (connectivity correlation 0.8). Module cyan displayed the least stability (Figure 1D).Evaluation of your Coexpression Module InteractionsWe analyzed the relationships among the 15 coexpression modules. Module eigengenes in this analysis had been defined as the IDO1 site initial principal component of a coexpression module matrix. Cluster evaluation was performed on these eigengenes (Figure 1E). The connectivity degree of eigengenes was determined to far better have an understanding of the interactions amongst the coexpression modules. The heatmap in Figure 1E showed the relatedness with the 15 coexpression modules identified by WGCNA, with red indicating close relatedness and blue indicating no relatedness. The outcomes demonstrated that the gene expression of every single module was mutually exclusive, indicating a high degree of scale independence.Coexpression Modules Considerably Correlated With Distinct Infection StatusTo recognize modules connected to primary and secondary infection, we calculated the correlations among module eigengenes and the infection status (Figure 1F). The modules have been selected employing a correlation p-value of 0.05 as a threshold. The genes inside the magenta (R = -0.61, p = 0.001) and yellow (R = 0.51, p = 0.01) modules are significantly positively or negatively correlated to the infection st.
