Abracci1 Infectivology and Clinical Trials Area, Type 1 Diabetes Centre, KIR3DL2 Proteins Formulation Children’s Hospital Bambino Ges Rome, Italy; 2The Cell Factory BVBA (Esperite NV), Niel, Belgium; 3Department of Women’s and Children’s Health SDB University of Padova, Padova, Italy; 4Department of Neuroscience, Children’ s Hospital Bambino Ges Rome, ItalyPF04.Differential interaction of platelet-derived extracellular vesicles with leukocyte subsets in human entire blood RenWeiss1; Marion Gr er2; Sabine Rauscher2; Birgit Fendl1; Tanja Eichhorn1; Michael Bernhard Fischer1; Andreas Spittler2; Viktoria Weber1 Department for Overall health Science and MMP-1 Proteins web Biomedicine, Danube University Krems, Krems, Austria; 2Medical University of Vienna, Vienna, AustriaBackground: There’s proof that extracellular vesicles (EVs) are mostly connected with granulocytes and monocytes, but scarcely with lymphocytes. Within this context, we studied the association of EVs with innate immune cells, particularly with monocyte subsets. Techniques: Association of EVs with immune cells was visualized by imaging flow cytometry and confocal microscopy. Monocyte subsets have been identified straight in complete blood according to their CD14 and CD16 expression as classical (CM; predominantly phagocytic; CD14 ++CD16-), intermediate (IM; phagocytic and pro-inflammatory; CD14++CD16+) and non-classical monocytes (NCM; mostly proinflammatory; CD14-CD16++) employing flow cytometry. The association of monocyte subsets with platelet EVs was detected applying lactadherin (LA) as marker of phosphatidylserine and CD41 as platelet marker. Along with the characterization in entire blood, we studied the association of platelet EVs with monocytes isolated from PBMCs by adverse depletion of non-monocytes. Results: Imaging flow cytometry and confocal microscopy confirmed the preferential interaction of platelet EVs with monocytes and granulocytes. The distribution of monocyte subsets in freshly drawn complete blood was 86.1 two.1 , 4.9 1.1 and 9.0 2.6 for CM, IM and NCM, respectively, and freshly isolated monocytes exhibited an practically identical distribution. Overnight resting, nevertheless, induced a significant shift towards IM (4.9 1.1 vs. 59.1 24.0). We located that 5.5 3.6 of all CM, 16.6 6.1 of all IM and three.5 two.1 of all NCM had been CD41+LA+, indicating their association with platelet EVs. Storage of entire blood induced an increase in monocyte-EV aggregates to 66.three 12.1 for CM, to 80.1 eight.7 for IM and to 28.4 11.1 for NCM, indicating the preferential association of EVs with CM and IM. Summary/Conclusion: Monocyte isolation and storage induce a shift towards IM. EVs exhibit differential interaction with monocyte subsets and are preferentially connected with CM and IM.Background: Mesenchymal stem cells (MSCs) exert their biological effects by way of secretion of extracellular vesicles (EVs). We previously showed that MSC-EVs have immunomodulatory properties on each human T and B cells. All-natural killer (NK) cells are important effectors inside our innate immunity but are in a position to influence the adaptive technique as well. They carry out the elimination of target cells through secretion of molecules including perforine/granzyme, cytokines and chemokines. Nevertheless, to date, our understanding regarding the immunomodulatory activity as well as the manage of MSCs over NK cell function is restricted. Within this study, we aim to investigate the immunomodulatory activity of clinical grade (CG) MSC-EVs on NK activities compared to parent MSCs. Approaches: Human umbilical cord-derived MSCs (.
