Is Gh1 for aqueous two-phase Fuscin Purity fermentation in 2 L Figure 3. Comparison of K value, PF and yield of BLIS from L. lactis Gh1 for aqueous two-phase fermentation in 2 L Chlorisondamine diiodide supplier bioreactor and Erlenmeyer flask. In the optimum circumstances of a ten (w/w) PEG2000, eight (w/w) dextran T500, pH 7 and flask. the optimum conditions of a 10 (w/w) (w/w) dextran T500, agitated at 200 rpm at 30 C. (……) ATPS in Erlenmeyer flask, and (___) ATPS in 2 L bioreactor. . ATPS in two L bioreactor. ATPS flask,The cell viability of L. lactis Gh1 in the course of homogeneous, and aqueous two-phase fermentation each in an Erlenmeyer flask in addition to a bench-scale bioreactor had been compared (Figure four). A larger cell viability was obtained from homogeneous fermentation when compared with that of extractive fermentation in both Erlenmeyer flask and also a bench-scale bioreactor. The presence of polymers (i.e., PEG and dextran) reduced the growth price of L. lactis Gh1.Fermentation 2021, 7,Figure three. Comparison of K value, PF and yield of BLIS from L. lactis Gh1 for aqueous two-phase fermentation in two L bioreactor and Erlenmeyer flask. At the optimum circumstances of a 10 (w/w) PEG2000, 8 (w/w) dextran T500, pH 7 and agitated at 200 rpm at 30 . (……) ATPS in Erlenmeyer flask, and (___) ATPS in two L bioreactor.14 ofTheThe cell viability of L. lactis Gh1 in the course of homogeneous,and aqueous two-phase fermencell viability of L. lactis Gh1 for the duration of homogeneous, and aqueous two-phase fermentationboth in an Erlenmeyer flask plus a a bench-scale bioreactor have been compared (Fig- four). tation both in an Erlenmeyer flask and bench-scale bioreactor have been compared (Figure ure A larger cellcell viability was obtained from homogeneous fermentation compared to of four). A higher viability was obtained from homogeneous fermentation in comparison with that thatextractive fermentation in bothboth Erlenmeyer flaska bench-scale bioreactor. The presence of extractive fermentation in Erlenmeyer flask and and also a bench-scale bioreactor. The presence of polymers (i.e., PEG and dextran) lowered therate of L. lactisof L. lactis Gh1. the of polymers (i.e., PEG and dextran) lowered the growth development price Gh1. However, However, the sustainable cells development wasin aqueous two-phasetwo-phase fermentation sustainable cells development was obtained obtained in aqueous fermentation in bioreactor in bioreactor make the repetitive fermentation scale feasible. achievable. make the repetitive fermentation in a substantial within a substantial scaleFigure four. Comparison with the cell viability of L. lactis Gh1 for homogeneous and aqueous two-phase fermentations in an Erlenmeyer flask as well as a two L bioreactor. The cells have been grown in the diverse fermentation environments (Erlenmeyer flask and batch bioreactor) and media, either with or with no polymer have been assayed and compared.The development pattern of L. lactis Gh1 in extractive fermentation exhibited precisely the same pattern as homogeneous culture in each bioreactor and shake flask. The exponential phase was started soon after 2 h to 6 h of fermentation plus the maximum viability was observed soon after 8 h when in flask the highest cell quantity shifted to h-10. Prolonged ATPS-fermentation inside the bioreactor preserves the high concentration of cells, when the cells enter the phase of death each within the flask ATPS and within the flask homogeneous culture. Maximum cell number of L. lactis Gh1 (1.09 109 CFU/mL) obtained in the homogeneous culture in flask was about 360 greater as when compared with both ATPS fermentation in flask (six.87 108 CFU/mL) and in bioreactor (5.43 108 CFU/mL). three.7. Repet.
