Ntrol group. Moreover, the 200 mg/kg BW CB group had
Ntrol group. Additionally, the 200 mg/kg BW CB group had decrease Sarizotan Autophagy p-P70S6K expression than the other three groups. The above outcomes indicate that CB increased the autophagy in a dose-dependent manner by enhancing Beclin 1 and LC3-II expressions via inhibiting the expressions of p-mTOR and p-P70S6K protein.Nutrients 2021, 13, 3652 Nutrients 2021, 13, x FOR PEER REVIEW6 of 13 6 ofFigure 2.two. Protein expressions ofthe livers in 3-month-old male SAMP8 mice fed with distinct diets for 12 weeks. (A) The mice fed with diverse diets for 12 weeks. (A) The Figure Protein expressions with the livers in 3-month-old gel image BDNF, p-CREB, total CREB, p-CAMK II, and -actin expressions. BDNF, (C) (C) p-CREB/total ratio, ratio, gel image BDNF, p-CREB, total CREB, p-CAMK II, and -actin expressions. (B) (B) BDNF, p-CREB/total CREBCREB and (D) p-CAMK II expressions from the the among diverse groups. The y The y axis quantified by the blot evaluation evaluation and (D) p-CAMK II expressions ofliver liver among distinctive groups. axis quantified by the western western blot protein of BDNF/-actin, p-CREB/CREB ratio, and p-CAMKII/-actin are expressed because the percentage of handle. Values are disprotein of BDNF/-actin, p-CREB/CREB ratio, and p-CAMKII/-actin are expressed as the percentage of manage. Values played as imply S.E.M. and were analyzed by one-way ANOVA (n = 6/group). p 0.05, p 0.005, p 0.001 are displayed as imply S.E.M. and had been analyzed by one-way ANOVA (n = 6/group). p 0.05, p 0.005, p 0.001 in comparison to the manage group. A = SAMP8 manage, B = 50 mg/kg BW/day CB, C = 100 mg/kg BW/day CB, D = 200 mg/kg compared to the control group. A = SAMP8 control, B = 50 mg/kg BW/day CB, C = one hundred mg/kg BW/day CB, D = 200 mg/kg BW/day CB. BW/day CB.3.3. CB Enhanced the Autophagic Expressions 3.4. CB Inhibited the Caspase-Independent Apoptosis Compared using the control group, the 200 mg/kg BW CB group had enhanced Sirt 1 In this study, we also evaluated the effects of CB around the caspase-independent apoptotic expression (Figure 3B). The Beclin 1 and LC3-II protein expressions within the liver are shown variables. When compared using the SAMP8 handle group, the one hundred and 200 mg/kg BW CB in Figures 3C,D, respectively. In comparison to the manage group, one hundred and 200 mg/kg BW CB groups showed a considerably reduce cleaved-PARP 1 and cleaved-PARP 1/PARP 1 ratio supplementations significantly enhanced Beclin 1 expression, whereas only the 200 mg/kg (Figure 4B,C). Moreover, the 200 mg/kg BW CB group had drastically reduced AIF BW CB group had considerably higher LC3-II expression than the manage group. Figures expression when compared to the control group (Figure 4D). These information suggest that CB 3E,F showed the p-mTOR and p-P70S6K protein expressions within the liver. The 100 and 200 enhanced the apoptosis reaction by downregulating the PARP 1 and AIF expressions. mg/kg BW CB groups had substantially decreased p-mTOR expressions in comparison with the control group. Furthermore, the 200 mg/kg BW CB group had decrease p-P70S6K expression than the other three groups. The above benefits indicate that CB increased the autophagy in a dose-dependent manner by enhancing Beclin 1 and LC3-II expressions through inhibiting the expressions of p-mTOR and p-P70S6K protein.Nutrients 2021, 13, 3652 Nutrients 2021, 13, x FOR PEER REVIEW7 of 13 7 ofFigure three. Protein expressions in the livers 3-month-old male SAMP8 mice fed with various diets for 12 weeks. (A) Figure 3. Protein expressions in the liver.
