Tity of every residue pair in every column, with green bars representing identical residues and missing bars highlighting regions where sequences differ. End gaps inside the alignments have solid green bars. Amino acids are shaded determined by similarity, with black representing identical residues in between the two sequences, grey representing equivalent residues, and white representing dissimilar residues. A = arrestin, B = cGMP gated channel alpha, C = cGMP gated channel beta, D = Goprotein alpha subunit, E = Gtprotein alpha subunit, F = Gprotein beta subunit, G = Gprotein gamma subunit, H = opsin, I = PDE alpha, J = PDE beta, K = protein kinase A, L = rhodopsin kinase. (PDF) Figure S3 Amino acid alignments of scallop circadian clock genes to identified homologs. Circadian clock gene sequences in the Placopecten magellanicus adult eye transcriptome have been translated and aligned to recognized homologs from Drosophila, mouse, or Crassostrea gigas. Alignments have been completed and exported from Geneious v. five.6 (www.geneious.com). The graph above the alignment represents imply pairwise identity of each residue pair in every column, with green bars representing identical residues and missing bars highlighting areas where the sequences differ. Finish gaps inside the alignments have solid green bars. Amino acids are shaded based on similarity, with black representingidentical residues among the two sequences, grey representing related residues, and white representing dissimilar residues. A = clock, B = cryptochrome, C = cycle, D = doubletime, E = period, F = timeless. (PDF)Table SList of phototransduction and circadian clock protein sequences utilised in blasts to identify homologs within every single scallop eye transcriptome. All protein sequences were downloaded from NCBI and blasted against the transcriptomes making use of Geneious v. five.5 (Biomatters). (XLSX)Table S2 Genes annotated with Gene Ontology (GO) terms related to neural processes, vision, or retina(l) inside the adult eye transctriptome of A. irradians. (XLSX) Table S3 Genes annotated with Gene Ontology (GO) terms associated with neural processes, vision, or retina inside the adult eye transcriptome of P. magellanicus. (XLSX) Table S4 List of genes identified in KEGG pathways related to light detection inside the adult eye transcriptomes of A. irradians and P. magellanicus. KEGG pathways had been identified working with the KEGG Automatic Annotation Server (www. genome.jp/tools/kaas). (XLSX)D-Glucose 6-phosphate (sodium) Technical Information AcknowledgmentsBrad Fleming kindly Acetylcholinesterase ache Inhibitors medchemexpress assisted with MATLAB scripts to organize data files and Fadi Towfic assisted together with the InParanoid analyses. Figure 1 was developed by Samantha Butler. The photo for Figure 1A was taken by Anita Krause. We thank Keithanne Mockaitis for her assistance with production of your Argopecten irradians transcriptome dataset. We also thank members of the D.C. Adams, N. Valenzuela, and J.M. Serb labs and 3 anonymous reviewers for their useful comments on preceding drafts of this manuscript.Author ContributionsConceived and developed the experiments: ANP JMS. Performed the experiments: ANP. Analyzed the data: ANP JMS. Contributed reagents/ materials/analysis tools: JMS. Wrote the paper: ANP JMS. Completed the scallop eye tissue dissections: ANP JMS.
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