Product Name: beta catenin Antibody
Species Reactivity: Human
Tested Applications: WB
Applications: Western Blot: 1:500~1:1000
User Note:
Predicted Molecular Weight: 92 kDa
Immunogen: beta-catenin antibody was raised against a peptide sequence around aa. 327~331 (C-I-M-R-T) derived from Human β-catenin .
Host Species: Rabbit
Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Physical State: Liquid
CAS NO.: 20575-57-9
Product: Calycosin
Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration: 1 mg/mL
Storage Conditions: Store antibody at -20˚C for up to one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CTNNB, MRD19, armadillo, FRAP, FRAP1, FRAP2, RAFT1, RAPT1, CTNNB1, CATNB, CTNB1, CTNNB
Accession NO.: NP_001091679.1
Protein Ino: 148233338
Official Symbol: CTNNB1
Geneid: 1499
Background: Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
PubMed ID:http://aac.asm.org/content/42/1/65.abstract
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Product Name: beta-Catenin Antibody
Species Reactivity: Human, Mouse
Tested Applications: Flow, IF, IHC, WB
Applications: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: A synthetic peptide from the middle of beta-Catenin / p120 protein was used as the immunogen.
Host Species: Rabbit
Purification: Protein A affinity chromatography
Physical State: Liquid
CAS NO.: 103-86-6
Product: Hydroxyamphetamine
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Catenin beta-1, Beta-catenin, CTNNB1, CTNNB
Accession NO.:
Protein Ino:
Official Symbol: CTNNB1
Geneid: 1499
Background: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, it is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution throughout the cytoplasm results in a diffuse cytoplasmic pattern. Staining with beta-catenin antibody and E-cadherin antibody helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
PubMed ID:http://aac.asm.org/content/42/2/475.abstract