Product Name: beta-Pix Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, ICC, WB
Applications: ELISA: (direct and indirect: 1:5,000-1:10,000). Immunocytochemistry: (1:1,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Recombinant beta-Pix SH3 domain.
Host Species: Rabbit
Purification:
Physical State: Liquid
CAS NO.: 304-84-7
Product: Etamivan
Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration: 1 mg/ml
Storage Conditions: Stable for at least 6 months after receipt when stored at -20˚C.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Rho Guanine Nucleotide Exchange Factor 7; PAK-interacting Exchange Factor beta; p85SPR; COOL-1
Accession NO.: Q9ES28
Protein Ino: 125987791
Official Symbol: Arhgef7
Geneid: 54126
Background: beta-PIX acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. It interacts with PAK kinases through the SH3 domain. It may function as a positive regulator of apoptosis and is involved in cell migration. It is expressed with high levels in the central nervous system.
PubMed ID:http://aac.asm.org/content/42/2/223.abstract
