Product Name: IL-7 Antibody
Species Reactivity: Human
Tested Applications: ELISA, Neut, WB
Applications: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-7 (1.00 ng/mL), a concentration of 0.016 0.03 μg/mL of this antibody is required. ELISA:To detect hIL-7 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 – 0.4 ng/well of recombinant hIL-7. Sandwich:To detect hIL-7 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-7 (XP-5197Bt) as a detection antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-7. Western Blot:To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-7 (human Interleukin-7).
Host Species: Rabbit
Purification: Anti-hIL-7 specific antibody was purified by affinity chromatography employing immobilized hIL-7 matrix.
Physical State: Lyophilized
CAS NO.: 84676-89-1
Product: Ferulic acid (sodium)
Buffer:
Concentration:
Storage Conditions: IL-7 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: IL-7Interleukin-7IL-7
Accession NO.: P13232
Protein Ino: 124354
Official Symbol: IL7
Geneid: 3574
Background: Iterleukin 7 is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL-7 stimulates the proliferation of CD4+/CD8+ cells.
PubMed ID:http://aac.asm.org/content/26/4/594.abstract
