Product Name: IL-6 Antibody [ITLN6]
Species Reactivity: Human, Mouse
Tested Applications: IF, IHC-P
Applications: Immunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Optimal dilution of the IL-6 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Recombinant mouse protein was used as the immunogen for the IL-6 antibody.
Host Species: Rat
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 6537-80-0
Product: L67
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Interleukin-6, IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IL6, IFNB2
Accession NO.:
Protein Ino:
Official Symbol: IL6
Geneid: 3569
Background: IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. It plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. It is involved in host defense, acute phase reactions, immune responses, and hematopoiesis.
PubMed ID:http://aac.asm.org/content/26/4/583.abstract
