Product Name: IL-3 Antibody
Species Reactivity: Human
Tested Applications: ELISA, Neut, WB
Applications: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-3 (1.0 ng/mL), a concentration of 0.38 – 0.57 μg/mL of this antibody is required.ELISA:To detect hIL-3 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-3.SandwichTo detect hIL-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-3 (XP-5185Bt) as a detection antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-3.Western Blot:To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-3 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-3 (human Interleukin-3).
Host Species: Rabbit
Purification: Anti-hIL-3 specific antibody was purified by affinity chromatography employing immobilized hIL-3 matrix.
Physical State: Lyophilized
CAS NO.: 58546-55-7
Product: GSK163090
Buffer:
Concentration:
Storage Conditions: IL-3 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: IL-3, MCGF, MULTI-CSFInterleukin-3, Hematopoietic growth factor, IL-3
Accession NO.: P08700
Protein Ino: 124330
Official Symbol: IL3
Geneid: 3562
Background: The pleiotropic IL-3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL-3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL-3 has also been shown to protect mast cells from undergoing apoptosis.
PubMed ID:http://aac.asm.org/content/26/3/414.abstract
