Product Name: IL-33 Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:Direct:To detect hIL-33 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-33.SandwichTo detect hIL-33 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-33 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-33. Western Blot:To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-33 (human IL-33). Human IL-33 specific antibody was purified by affinity chromatography and then biotinylated.
Host Species: Rabbit
Purification:
Physical State: Lyophilized
CAS NO.: 364-98-7
Product: Benzetimide (hydrochloride)
Buffer:
Concentration:
Storage Conditions: IL-33 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26, RP11-575C20.2, NFHEV, Interleukin-33, Interleukin-1 family member 11, IL-33
Accession NO.: O95760
Protein Ino: 68565855
Official Symbol: IL33
Geneid: 90865
Background: IL-33 is a cytokine which belongs to the IL-1 superfamily, and it induces helper T cells to produce type 2 cytokines. This cytokine was previously named NF-HEV nuclear factor (NF) in high endothelial venules (HEVs), as it was originally identified in these specialized cells. IL-33 mediates its biological effects by interacting with the receptors ST2 and IL-1 Receptor Accessory Protein, activating intracellular molecules in the NF-kappaB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-4, IL-5 and IL-13) from polarized Th2 cells. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the the severe pathological changes observed in mucosal organs following administration of IL-33.
PubMed ID:http://aac.asm.org/content/26/3/428.abstract
