Product Name: IL-33 Antibody [IL33068A]
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA: (direct or indirect: 1:2,000-1:5,000). Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Recombinant human IL-33.
Host Species: Mouse
Purification:
Physical State: Liquid
CAS NO.: 3562-99-0
Product: Baicalin
Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration: 1 mg/ml
Storage Conditions: Stable for at least 1 year after receipt when stored at -20˚C.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Interleukin-33; IL-1F11; NF-HEV
Accession NO.: NP_254274
Protein Ino: 15559209
Official Symbol: IL33
Geneid: 90865
Background: Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1(MAPK3)/ERK2(MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.
PubMed ID:http://aac.asm.org/content/26/4/446.abstract
