Product Name: IL-2 Antibody [5B9-2-1]
Species Reactivity: Mouse
Tested Applications: ELISA, Neut, WB
Applications: ELISA:Sandwich:In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μg/mL. Western Blot:To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 μg/mL of this antibody is required.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: E.coli derived Recombinant Murine IL-2
Host Species: Rat
Purification: This IgG2Ak antibody was purified from cell culture by Protein A affinity chromatography.
Physical State: Lyophilized
CAS NO.: 3759-92-0
Product: 6-Quinoxalinecarboxylic acid, 2,3-bis(bromomethyl)-
Buffer:
Concentration:
Storage Conditions: The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Interleukin 2Interleukin-2, T-cell growth factor, IL-2, TCGF, lymphokine
Accession NO.: P60568
Protein Ino: 45593462
Official Symbol: IL2
Geneid: 3558
Background:
PubMed ID:http://aac.asm.org/content/26/3/300.abstract
