Product Name: IL-27 Antibody
Species Reactivity: Human, Mouse
Tested Applications: ELISA, ICC, WB
Applications: IL-27 antibody can be used for the detection of IL-27 by Western blot at 1 – 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 27 kDa Observed: 29 kDa
Immunogen: IL-27 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-27.The immunogen is located within the first 50 amino acids of IL-27.
Host Species: Rabbit
Purification: IL-27 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 102841-42-9
Product: Maytansinol
Buffer: IL-27 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: IL-27 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: IL-27 Antibody: p28, IL30, IL-27, IL27A, IL-27A, IL27p28, Interleukin-27 subunit alpha, p28, IL-27 subunit alpha
Accession NO.: NP_663634
Protein Ino: 28416913
Official Symbol: IL27
Geneid: 246778
Background: IL-27 Antibody: Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.
PubMed ID:http://aac.asm.org/content/26/3/405.abstract
