Product Name: IL-1a Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:To detect Rat IL-1-α by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 – 0.30 μg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant Rat IL-1-α. Sandwich:To detect hIL-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-1α (XP-5174) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-1α.Western Blot:To detect Rat IL-1-α by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1-α is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat IL-1-alpha (Rat IL-1-alpha).
Host Species: Rabbit
Purification: Anti-Rat IL-1-alpha specific antibody was purified by affinity chromatography and then biotinylated.
Physical State: Lyophilized
CAS NO.: 898839
Product: MUT056399
Buffer:
Concentration:
Storage Conditions: IL-1a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: IL1, IL-1A, IL1F1, IL1-ALPHA, Interleukin-1 alpha, Hematopoietin-1, IL-1 alpha
Accession NO.: P01583
Protein Ino: 124297
Official Symbol: IL1A
Geneid: 3552
Background:
PubMed ID:http://aac.asm.org/content/26/2/250.abstract
