Product Name: IL-1RA Antibody
Species Reactivity: Human
Tested Applications: ELISA, Neut, WB
Applications: ELISA:Sandwich:To detect Human IL-1RA by sandwich ELISA (using 100μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Polyclonal Biotinylated Anti-Human IL-1RA (38-139) as a detection antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IL-1RA. Western Blot:To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-1RA (50.0 ng/mL) and Human IL-1a(50.0 pg/mL), a concentration of 2.5-3.7 μg/mL of this antibody is required.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: E.coli derived Recombinant Human IL-1Ra
Host Species: Rabbit
Purification: IL-1RA specific antibody was purified by affinity chromatography employing an immobilized Human IL-1RA matrix.
Physical State: Lyophilized
CAS NO.: 3863-59-0
Product: Ribocil
Buffer:
Concentration:
Storage Conditions: The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: DIRA, IRAP, IL1F3, IL1RA, MVCD4, IL-1RN, IL-1ra, IL-1ra3, ICIL-1RA, Interleukin-1 receptor antagonist protein
Accession NO.: P18510
Protein Ino: 124312
Official Symbol: IL1RN
Geneid: 3557
Background:
PubMed ID:http://aac.asm.org/content/26/2/275.abstract
