Product Name: IL-17F Antibody
Species Reactivity: Human
Tested Applications: ELISA, Neut, WB
Applications: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17F (50.0 ng/mL), a concentration of 0.9-1.2 μg/mL of this antibody is required. ELISA:Indirect:To detect hIL-17F by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-17F.SandwichTo detect hIL-17F by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human IL-17F as a detection antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-17F. Western Blot:To detect hIL-17F by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17F is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17F. Human IL-17F specific antibody was purified by affinity chromatography employing immobilized hIL-17F matrix.
Host Species: Rabbit
Purification:
Physical State: Lyophilized
CAS NO.: 51-79-6
Product: Tubastatin-A
Buffer:
Concentration:
Storage Conditions: The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: ML1, ML-1, CANDF6, IL-17FInterleukin-17F, Cytokine ML-1, IL-17F
Accession NO.: Q96PD4
Protein Ino: 239938888
Official Symbol: IL17F
Geneid: 112744
Background:
PubMed ID:http://aac.asm.org/content/26/2/204.abstract
