Product Name: IKK beta Antibody
Species Reactivity: Human
Tested Applications: ELISA, ICC, IF, WB
Applications: IKK beta antibody can be used for detection of IKK beta by Western blot at 0.5 μg/mL. A 87 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 87 kDa
Immunogen: IKK beta antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IKK beta (Genbank accession NoO14920), which differs from corresponding murine sequence by one amino acid.The immunogen is located within amino acids 650 – 700 of IKK beta.
Host Species: Rabbit
Purification: IKK beta Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 3511-16-8
Product: GSK2838232
Buffer: IKK beta Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
Storage Conditions: IKK beta antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: IKK beta Antibody: IKK2, IKKB, IMD15, NFKBIKB, IKK-beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B kinase 2, I-kappa-B-kinase beta
Accession NO.: O14920
Protein Ino: 14285497
Official Symbol: IKBKB
Geneid: 3551
Background: IKK beta Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for NF-κB activation. IKKβ phosphorylates both IκB-alpha and IκB-beta. IKKβ is expressed in variety of human tissues.
PubMed ID:http://aac.asm.org/content/25/6/760.abstract
