Product Name: IKK a (phospho Thr23) Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: IHC, WB
Applications: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
User Note:
Predicted Molecular Weight: 85 kDa
Immunogen: IKK a (Phospho-Thr23) antibody was raised against a peptide sequence around phosphorylation site of threonine 23 (L-G-T (p) -G-G) derived from Human IKK α.
Host Species: Rabbit
Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Physical State: Liquid
CAS NO.: 347379-29-7
Product: DPC-681
Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration: 1 mg/mL
Storage Conditions: Store antibody at -20˚C for up to one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, CTNNB, OK/SW-cl.35, PRO2286, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK1
Accession NO.: NP_001269.3
Protein Ino: 62241001
Official Symbol: CHUK
Geneid: 1147
Background: Acts as part of the IKK complex in the conventional pathway of NF-κ-B activation and phosphorylates inhibitors of NF-κ-B thus leading to the dissociation of the inhibitor/NF-κ-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-κ-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-κ-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates Ser-10 of histone H3 at NF-κ-B-regulated promoters during inflammatory responses triggered by cytokines.
PubMed ID:http://aac.asm.org/content/25/6/742.abstract
