Product Name: IKBKE Antibody [6B4B5]
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: Western Blot:1:500 – 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Purified truncated recombinant IKBKE-His (aa1-257) is expressed in E. Coli strain BL21 (DE3).
Host Species: Mouse
Purification:
Physical State:
CAS NO.: 186139-09-3
Product: Indirubin-3-monoxime
Buffer:
Concentration:
Storage Conditions: IKBKE monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: IKKE, IKKI, KIAA0151, Inducible I kappa-B kinase, I-kappa-B kinase epsilon, IKKE, IKK-E, IKK-i
Accession NO.: Q14164
Protein Ino: 14548079
Official Symbol: IKBKE
Geneid: 9641
Background: Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon.The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB. Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degra-dation of IκB. IκB kinase α(IKKα), previously designated CHUK, interacts with IκB-αand specifically phosphorylates IκB-αon the sites that trigger its degradation, serines 32 and 36. The functional IKK complex contains three subunits, IKKα, IKKβand IKKγ(also designated NEMO), and each appear to make essential contributions to IκB phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKKαand IKKβ. IKK-i is pri-marily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNFα, IL-1 and IL-6. Overexpression of IKK-i was shown to result in phosphorylation of IκBαon Ser32 and Ser36, and in NFκB activation, suggesting that IKK-i may act as an IκB kinase in the immune system.
PubMed ID:http://aac.asm.org/content/25/6/725.abstract
