Product Name: IFN-gamma Antibody [1.1-5A9-2D05]
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human IFN-γ when used with our biotinylated antigen affinity purified anti-human IFN-γ (XP-5155Bt) as the detection antibody at a concentration of approximately 2.0-4.0 μg/mL.Western Blot:To detect hIFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-γ is 1.0-3.0 ng/lane, under non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: The monoclonal antibody was produced in BALB/c x ICR F1 mice using recombinant IFN-gamma as the immunizing antigen.
Host Species: Mouse
Purification: This IgG1k antibody was purified on a Protein A affinity column.
Physical State: Lyophilized
CAS NO.: 81161-17-3
Product: Evatanepag
Buffer:
Concentration:
Storage Conditions: IFN-γ lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 – 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: AF1, Interferon gamma receptor , Interferon gamma transducerInterferon gamma, Immune interferon, IFN-gamma, IFG, IFI
Accession NO.: P01579
Protein Ino: 124479
Official Symbol: IFNG
Geneid: 3458
Background:
PubMed ID:http://aac.asm.org/content/25/2/286.abstract