Product Name: IDO Antibody
Species Reactivity: Human
Tested Applications: ELISA, Flow, ICC, WB
Applications: ELISA: (direct and indirect: 1:2,000-1:10,000). Flow Cytometry: (1:200-1:500). Immunocytochemistry: (1:500) . Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Recombinant human IDO.
Host Species: Rabbit
Purification:
Physical State: Liquid
CAS NO.: 555-77-1
Product: SGI-7079
Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration: 1 mg/ml
Storage Conditions: Stable for at least 6 months after receipt when stored at -20˚C.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Indoleamine 2,3-dioxygenase; INDO
Accession NO.: NP_002155.1
Protein Ino: 4504577
Official Symbol: IDO1
Geneid: 3620
Background: IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.
PubMed ID:http://aac.asm.org/content/25/1/140.abstract
