Product Name: Huntingtin Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC, WB
Applications: Huntingtin antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 1:500, immunofluorescence, and immunoprecipitation.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Huntingtin antibody was raised against amino acids 416-424 from the C-Terminus of Huntingtin (Human).
Host Species: Rabbit
Purification: Immunoaffinity Chromatography
Physical State: Liquid
CAS NO.: 213997-73-0
Product: alpha-Hederin
Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration: 1 mg/ml
Storage Conditions: Antibody should be stored long term (months) at -20 ˚C and short term (weeks) at 4 ˚C. As with all antibodies avoid freeze/thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: HTT, Huntingtin, Huntington disease protein, IT15, HD, HD protein
Accession NO.: P42858
Protein Ino: 296434520
Official Symbol: HTT
Geneid: 3064
Background: Huntingtin (also known as Huntingtons disease protein, Htt and HD protein) is the protein product of a disease gene linked to Huntingtons disease, a neuro-degenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product (see partial protein sequence below). The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntingtons disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
PubMed ID:http://aac.asm.org/content/24/5/735.abstract
