Product Name: Heregulin-beta1 Antibody
Species Reactivity: Human
Tested Applications: ELISA, Neut, WB
Applications: Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hHeregulin-B1 (1.0 ng/mL), a concentration of 0.1-1.1 μg/mL of this antibody is required.ELISA:In a sandwich ELISA To detect hHeregulin-B1 (using 100 uL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction withour biotinylated Anti-Human hHeregulin-B1as a detection antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hHeregulin-B1. Western Blot:To detect hHeregulin-B1 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHeregulin-B1 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human Heregulin-B1. Human Heregulin-B1 specific antibody was purified by affinity chromatography employing an immobilized Human Heregulin-B1 matrix.
Host Species: Rabbit
Purification:
Physical State: Lyophilized
CAS NO.: 160098-96-4
Product: (R)-(-)-Phenylephrine
Buffer:
Concentration:
Storage Conditions: The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: GGF, HGL, HRG, NDF, ARIA, GGF2, HRG1, HRGA, SMDF, MST131, GGF, Pro-neuregulin-1, membrane-bound isoform, Acetylcholine receptor-inducing activity, Pro-NRG1
Accession NO.: Q02297
Protein Ino: 9297018
Official Symbol: NRG1
Geneid: 3084
Background:
PubMed ID:http://aac.asm.org/content/22/2/218.abstract
