Product Name: HTR1B Antibody
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: HTR1B antibody can be used for detection of HTR1B by ELISA at 1:12500. HTR1B antibody can be used for detection of HTR1B by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 44 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HTR1B.
Host Species: Rabbit
Purification: Antibody is purified by protein A chromatography method.
Physical State: Lyophilized
CAS NO.: 856867-55-5
Product: PHCCC
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store HTR1B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: HTR1B, S12, 5-HT1B, HTR1D2, HTR1DB, 5-HT1DB
Accession NO.: NP_000854
Protein Ino: 4504533
Official Symbol: HTR1B
Geneid: 3351
Background: The pathophysiology of depression remains enigmatic, although abnormalities that involve serotonin signaling have been implicated. The serotonin 1B receptor [5-hydroxytryptamine (5-HT1B) receptor] interacts with p11. p11 increases localization of 5-HT1B receptors at the cell surface. p11 is increased in rodent brains by antidepressants or electroconvulsive therapy, but decreased in an animal model of depression and in brain tissue from depressed patients. Overexpression of p11 increases 5-HT1B receptor function in cells and recapitulates certain behaviors seen after antidepressant treatment in mice. p11 knockout mice exhibit a depression-like phenotype and have reduced responsiveness to 5-HT1B receptor agonists and reduced behavioral reactions to an antidepressant.
PubMed ID:http://aac.asm.org/content/24/4/597.abstract
