Product Name: HPRT Antibody [1F8D11]
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: Western Blot:Dilution 1:500 – 1:2,000 ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Ni-NTA purified truncated recombinant HPRT expressed in E. Coli strain BL21 (DE3).
Host Species: Mouse
Purification:
Physical State:
CAS NO.: 1014-70-6
Product: Triethanolamine
Buffer:
Concentration:
Storage Conditions: HPRT monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: HPRT, Hypoxanthine-guanine phosphoribosyltransferase, HGPRT
Accession NO.: P00492
Protein Ino: 123497
Official Symbol: HPRT1
Geneid: 3251
Background: The HPRT1 gene provides instructions for making an enzyme called hypoxanthine phosphoribosyltransferase 1. This enzyme allows cells to recycle purines, some of the building blocks of DNA and its chemical cousin RNA.The enzyme hypoxanthine-guanine phosphoribosyltrasferase (E.C.2.4.2.8., HPRT) plays a crucial role in uric acid synthesis and purine metabolism.This enzyme catalyzes the conversion of hypoxanthine and guanine to inosine monophosphate (IMP) and guanosine monophosphate (GMP), respectively, and uses phosphoribosylpyrophosphate (PRPP) as a cosubstrate and as a source of energy . This pathway is also known as the purine salvage pathway because it allows cells to reuse purine compounds to build DNA and RNA.
PubMed ID:http://aac.asm.org/content/23/5/784.abstract
