Product Name: HPRT1 Antibody
Species Reactivity: Dog, Human, Mouse, Rat
Tested Applications: ELISA, WB
Applications: HPRT1 antibody can be used for detection of HPRT1 by ELISA at 1:12500. HPRT1 antibody can be used for detection of HPRT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 24 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HPRT1.
Host Species: Rabbit
Purification: Antibody is purified by peptide affinity chromatography method.
Physical State: Lyophilized
CAS NO.: 898563-00-3
Product: CCT245737
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store HPRT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: HPRT1, HGPRT, HPRT
Accession NO.: NP_000185
Protein Ino: 4504483
Official Symbol: HPRT1
Geneid: 3251
Background: HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate.HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).
PubMed ID:http://aac.asm.org/content/23/6/799.abstract
