Product Name: HIF3A Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC, WB
Applications: HIF3A antibody can be used for detection of HIF3A by ELISA at 1:312500. HIF3A antibody can be used for detection of HIF3A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 72 kDa, 66 kDa, 72 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HIF3A.
Host Species: Rabbit
Purification:
Physical State: Lyophilized
CAS NO.: 153-18-4
Product: DHMEQ (racemate)
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store HIF3A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: HIF3A, IPAS, MOP7, PASD7, HIF-3A, bHLHe17
Accession NO.: NP_690007
Protein Ino: 23065535
Official Symbol: HIF3A
Geneid: 64344
Background: HIF3A is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression.The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. At least three transcript variants encoding three different isoforms have been found for this gene.
PubMed ID:http://aac.asm.org/content/22/4/579.abstract
