Product Name: GST Antibody [6G9B9]
Species Reactivity: GST
Tested Applications: ELISA, WB
Applications: Western Blot:Dilution 1:2000 – 5,000ELISA:Proposed dilution 1:10,000.Determining optimal working dilutions by titration test.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Ni-NTA purified recombinant GST expressed in E. Coli strain M15.
Host Species: Mouse
Purification: Antibody is purified by protein A affinity chromatography.
Physical State:
CAS NO.: 1339058-04-6
Mivebresib
Buffer: Purified antibody in PBS containing 0.03% sodium azide
Concentration:
Storage Conditions: GST monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: gst, b1635, JW1627, GST B1-1
Accession NO.: P0A9D2
Protein Ino: 71159393
Official Symbol: gst
Geneid: 12931264
Background: Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
PubMed ID:http://aac.asm.org/content/20/4/481.abstract
