Product Name: GRO/MGSA Antibody
Species Reactivity: Human
Tested Applications: ELISA, Neut, WB
Applications: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO/MGSA 100 ng/mL), a concentration of 1.5 – 3.0 μg/mL of this antibody is required. ELISA:To detect hGRO/MGSA by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 – 0.4 ng/well of recombinant hGRO/MGSA. Sandwich: To detect hGRO/MGSA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human GRO/MGSA (XP-5147Bt) as a detection antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hGRO/MGSA. Western Blot:To detect hGRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO/MGSA is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGRO/MGSA (human GRO/MGSA).
Host Species: Rabbit
Purification: Anti-hGRO/MGSA specific antibody was purified by affinity chromatography employing immobilized hGRO/MGSA matrix.
Physical State: Lyophilized
CAS NO.: 6754-58-1
HG6-64-1
Buffer:
Concentration:
Storage Conditions: GRO/MGSA antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: FSP, GRO1, GROa, MGSA, NAP-3, SCYB1, MGSA-a, GRO, GROA, Growth-regulated alpha protein, C-X-C motif chemokine 1
Accession NO.: P09341
Protein Ino: 121622
Official Symbol: CXCL1
Geneid: 2919
Background:
PubMed ID:http://aac.asm.org/content/20/2/197.abstract
