Product Name: GRO/KC Antibody (biotin)
Species Reactivity: Rat
Tested Applications: ELISA, WB
Applications: ELISA:To detect rat GRO/KC by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 – 0.30 μg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat GRO/KC. Sandwich:To detect Rat GRO/KC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat GRO/KC (XP-5146) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat GRO/KC.Western Blot:To detect rat GRO/KC by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat GRO/KC is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat GRO/KC (rat GRO/KC).
Host Species: Rabbit
Purification: Anti-rat GRO/KC specific antibody was purified by affinity chromatography and then biotinylated.
Physical State: Lyophilized
CAS NO.: 34208-98-5
ARRY-520
Buffer:
Concentration:
Storage Conditions: GRO/KC antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: Gro1, CINC-1, Cinc1, Gro, Scyb1, Growth-regulated alpha protein, C-X-C motif chemokine 1
Accession NO.: P14095
Protein Ino: 1170021
Official Symbol: Cxcl1
Geneid: 81503
Background:
PubMed ID:http://aac.asm.org/content/20/2/190.abstract
