Product Name: CD20 Antibody [93-1B3]
Species Reactivity: Human
Tested Applications: Flow, Func, IF, WB
Applications: Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlFunctional Testing (Order Ab without BSA & Azide)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD20 antibody to be titered up or down for optimal performance.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Stimulated human leukocytes were used as the immunogen for this CD20 antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 122008-78-0
Product: Cyhalofop
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20
Accession NO.:
Protein Ino:
Official Symbol: MS4A1
Geneid: 931
Background: This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). The antibody recognizes the extracellular domain of the protein. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression.
PubMed ID:http://aac.asm.org/content/34/4/524.abstract
