Product Name: Biotin Antibody [SPM375]
Species Reactivity: All species
Tested Applications: CM, Flow, Func, IF, IHC, WB
Applications: Confocal MicroscopyFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlIn situ Nucleic Acid HybridizationWestern: blot 1-3 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 25013-16-5
Product: Butylhydroxyanisole
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names:
Accession NO.:
Protein Ino:
Official Symbol:
Geneid:
Background: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
PubMed ID:http://aac.asm.org/content/42/3/640.abstract
