Product Name: Biotin Antibody [Hyb-8]
Species Reactivity: All species
Tested Applications: CM, Flow, IF, IHC, WB
Applications: IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIn situ Nucleic Acid HybridizationWB: 2-5 ug/mlConfocal MicroscopyThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this Biotin antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 25990-60-7
Product: DL-Xylose
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names:
Accession NO.:
Protein Ino:
Official Symbol:
Geneid:
Background: This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Biotin antibody may be substituted to decrease this noise.
PubMed ID:http://aac.asm.org/content/42/3/618.abstract
