Product Name: Beclin 2 Antibody
Species Reactivity: Human
Tested Applications: ELISA, IF, IHC-P, WB
Applications: Beclin 2 antibody can be used for detection of Beclin 2 by Western blot at 1 – 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 47 kDa Observed: 55 kDa
Immunogen: Beclin 2 antibody was raised against a 16 amino acid peptide near the amino terminus of human Beclin 2.The immunogen is located within amino acids 30 – 80 of Beclin 2.
Host Species: Rabbit
Purification: Beclin 2 antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 345909-26-4
Product: Taurocholic Acid (sodium hydrate)
Buffer: Beclin 2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: Beclin 2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: BECN2, BECN1L1, Beclin-1-like protein 1, Beclin 1 autophagy related pseudogene 1, BECN1P1
Accession NO.: NP_001277622
Protein Ino: 595582373
Official Symbol: BECN1P1
Geneid: 441925
Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2). Beclin 2 is a mammalian specific homolog of the autophagy protein Beclin 1 (3). Like Beclin 1, Beclin 2 interacts with Bcl-2 and class III PI3K complex components. However, Beclin 2 functions in an additional lysosomal degradation pathway and is required for ligand-induced endolysosomal degradation of several G protein-coupled receptors (3). Beclin 2 is also required for agonist-induced lysosome-mediated degradation of EGFR in lung cancer cells, suggesting that it may also play a role in regulating other intracellular signaling pathways (4).
PubMed ID:http://aac.asm.org/content/42/1/173.abstract
