Product Name: Bcl-X Antibody [SPM165]
Species Reactivity: Human, Mouse, Pig, Rat
Tested Applications: Flow, IF, IHC, WB
Applications: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The optimal dilution of the anti-Bcl-X antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Amino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this anti-Bcl-X antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 41354-29-4
Product: Cyproheptadine (hydrochloride sesquihydrate)
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Bcl-xL Antibody: BCLX, BCL2L, BCLXL, BCLXS, Bcl-X, bcl-xL, bcl-xS, PPP1R52, BCL-XL/S, BCLX, Bcl-2-like protein 1, Apoptosis regulator Bcl-X, Bcl2-L-1
Accession NO.:
Protein Ino:
Official Symbol: BCL2L1
Geneid: 598
Background: Recognizes a protein of 27kDa, identified as the Bcl-X protein. This mAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.
PubMed ID:http://aac.asm.org/content/53/12/5236.abstract
