Product Name: B-NGF Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:To detect hβ-NGF by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 – 0.30 μg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hβ-NGF. Western Blot:To detect hβ-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ-NGF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hβ-NGF (human β-NGF).
Host Species: Rabbit
Purification: Anti-hβ-NGF specific antibody was purified by affinity chromatography and then biotinylated.
Physical State: Lyophilized
CAS NO.: 9087-70-1
Product: Aprotinin
Buffer:
Concentration:
Storage Conditions: B-NGF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: NGFB, HSAN5, Beta-NGF, NGFB, Beta-nerve growth factorBeta-NGF
Accession NO.: P01138
Protein Ino: 90110037
Official Symbol: NGF
Geneid: 4803
Background:
PubMed ID:http://aac.asm.org/content/53/9/3908.abstract