Product Name: B-NGF Antibody
Species Reactivity: Human
Tested Applications: ELISA, Neut, WB
Applications: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hβ-NGF (1 ng/mL), a concentration of 0.05 – 0.08 μg/mL of this antibody is required. ELISA:To detect hβ-NGF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 – 0.4 ng/well of recombinant hβ-NGF. Western Blot:To detect hβ-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ-NGF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hβ-NGF (Human beta-Nerve Growth Factor).
Host Species: Rabbit
Purification: Anti-hβ-NGF specific antibody was purified by affinity chromatography employing immobilized hβ-NGF matrix.
Physical State: Lyophilized
CAS NO.: 204656-20-2
Product: Liraglutide
Buffer:
Concentration:
Storage Conditions: B-NGF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: NGFB, HSAN5, Beta-NGF, NGFB, Beta-nerve growth factorBeta-NGF
Accession NO.: P01138
Protein Ino: 90110037
Official Symbol: NGF
Geneid: 4803
Background:
PubMed ID:http://aac.asm.org/content/53/9/3832.abstract