Product Name: BD-4 Antibody
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:Indirect:To detect hBD-4 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBD-4.SandwichTo detect hBD-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human BD-4 as a detection antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBD-4. Western Blot:To detect hBD-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-4 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-4 (human Beta Defensin-4). Human BD-4 specific antibody was purified by affinity chromatography employing immobilized hBD-4 matrix.
Host Species: Rabbit
Purification:
Physical State: Lyophilized
CAS NO.: 906-33-2
Product: Neochlorogenic acid
Buffer:
Concentration:
Storage Conditions: BD-4 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: BD-4, DEFB4, hBD-4, DEFB-4, DEFB104, DEFB104BBeta-defensin 104, Beta-defensin 4, BD-4
Accession NO.: Q8WTQ1
Protein Ino: 118572637
Official Symbol: DEFB104A
Geneid: 140596
Background:
PubMed ID:http://aac.asm.org/content/53/12/5122.abstract