Product Name: BD-3 Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:Direct:To detect hBD-3 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBD-3.SandwichTo detect hBD-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human BD-3 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBD-3. Western Blot:To detect hBD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-3 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-3. Human BD-3 specific antibody was purified by affinity chromatography and then biotinylated.
Host Species: Rabbit
Purification:
Physical State: Lyophilized
CAS NO.: 27215-14-1
Product: Neoandrographolide
Buffer:
Concentration:
Storage Conditions: BD-3 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: BD-3, HBD3, HBP3, DEFB3, DEFB103, BD3, DEFB103BBeta-defensin 103, Beta-defensin 3, BD-3
Accession NO.: P81534
Protein Ino: 17372441
Official Symbol: DEFB103B
Geneid: 55894
Background:
PubMed ID:http://aac.asm.org/content/53/12/5074.abstract
