Product Name: BCKDK Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, WB
Applications: BCKDK antibody can be used for detection of BCKDK by ELISA at 1:62500. BCKDK antibody can be used for detection of BCKDK by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 43 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BCKDK.
Host Species: Rabbit
Purification: Antibody is purified by peptide affinity chromatography method.
Physical State: Lyophilized
CAS NO.: 56238-63-2
Product: Cefuroxime (sodium)
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store BCKDK antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: BCKDK, BDK, BCKDKD
Accession NO.: NP_005872
Protein Ino: 171906589
Official Symbol: BCKDK
Geneid: 10295
Background: BCKDK belongs to the PDK/BCKDK protein kinase family. It contains 1 histidine kinase domain. BCKDK catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. BCKDK is the key enzyme that regulates the activity state of the BCKD complex.
PubMed ID:http://aac.asm.org/content/53/11/4907.abstract
