Product Name: BARD1 Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC
Applications: BARD1 antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 10 μg/mL, immunofluorescence, and immunoprecipitation.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Amino acids 108 to 124 of human BARD1
Host Species: Rabbit
Purification: Protein G Column
Physical State: Liquid
CAS NO.: 1254205-52-1
Product: RQ-00203078
Buffer: PBS, 0.1% sodium azide.
Concentration:
Storage Conditions: Antibody should be stored long term (months) at -20 ˚C and short term (weeks) at 4 ˚C. As with all antibodies avoid freeze/thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: BARD1, BARD-1, BRCA1 associated RING domain 1
Accession NO.: Q99728
Protein Ino: 116241265
Official Symbol: BARD1
Geneid: 580
Background: BARD1 interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, BARD1 shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. The BARD1 protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. BARD1 may be the target of oncogenic mutations in breast or ovarian cancer.
PubMed ID:http://aac.asm.org/content/53/10/4537.abstract
