Product Name: BAFF Antibody [Buffy-2]
Species Reactivity: Human, Mouse
Tested Applications: Flow, IHC, WB
Applications: BAFF antibody can be used to recognize membrane-bound and soluble human and mouse (weak) BAFF by Flow Cytometry, Immunohistochemistry (frozen sections, paraffin sections) and Western Blot. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line). Cells were incubated on ice for 30min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μg/mL of MAb to BAFF (Buffy-2) (FITC). After washing, cells were analyzed by flow cytometry. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution. Please inquire for complete protocol..
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: BAFF (monoclonal Buffy-2) antibody was raised against recombinant human soluble BAFF (aa. 83-285).
Host Species: Rat
Purification: Anti BAFF (monoclonal Buffy-2) antibody was purified to ≥95% in SDS-PAGE.
Physical State: Liquid
CAS NO.: 52-68-6
Product: Metrifonate
Buffer: PBS containing 0.02% sodium azide
Concentration:
Storage Conditions: BAFF antibody can be stored at 4˚C, stable for one year.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: BAFF, BLYS, TALL1, TNFSF20, ZTNF4, UNQ401/PRO738, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, BLyS, DTL, BAFF, CD257, THANK, TALL-1
Accession NO.: Q9Y275
Protein Ino: 13124573
Official Symbol: TNFSF13B
Geneid: 10673
Background:
PubMed ID:http://aac.asm.org/content/53/10/4414.abstract
