Product Name: BAFF Antibody [2B]
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 5.0-6.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human BAFF when used with our biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 μg/mL. Western Blot:To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: The monoclonal antibody was produced in BALB/c mice using recombinant human BAFF as the immunizing antigen.
Host Species: Mouse
Purification: This IgG1/K antibody was purified from ascites fluid on a Protein A chromatography column.
Physical State: Lyophilized
CAS NO.: 1021868-92-7
Product: ZM39923 (hydrochloride)
Buffer:
Concentration:
Storage Conditions: BAFF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 – 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: B cell Activating Factor , BAFF, BLYS, TALL1, TNFSF20, ZTNF4, UNQ401/PRO738, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, BLyS, DTL, CD257, THANK, TALL-1
Accession NO.: Q9Y275
Protein Ino: 13124573
Official Symbol: TNFSF13B
Geneid: 10673
Background:
PubMed ID:http://aac.asm.org/content/53/10/4298.abstract