Product Name: Arrestin Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IHC-P, IP, WB
Applications: ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 40kDa band observed in Human Brain lysates (calculated MW of 46.1kDa according to NP_004304.1 and 44.4 according to NP_945355.1). Recommended concentration: 0.3-1μg/ml. An anonymous customer found positive results in WB on Rat aorti Immunohistochemistry: In paraffin embedded Human Cerebellum shows mainly nuclear staining of purkinje cells and neuronal cells in the Molecular Layer. Recommended concentration, 4-6μg/ml. Immunoprecipitation: This antibody has been successfully used in IP from rat leukocytes as described in the following paper: Zakrzewicz et al, Immunobiology. 2011 Jul;216(7):854-61; PMID: 21193245.
User Note:
Predicted Molecular Weight:
Immunogen: Arrestin antibody was raised against an 11 amino acid synthetic peptide near the internal region of Arrestin.
Host Species: Goat
Purification: Arrestin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 1446700-26-0
Product: CO-1686 (hydrobromide)
Buffer: Arrestin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: ARRB2, arrestin, beta 2, HGNC:712, ARB2, ARR2, DKFZp686L0365, arrestin beta 2, beta-arrestin 2
Accession NO.: NP_004304.1, NP_945355.1
Protein Ino: 4757780
Official Symbol: ARRB2
Geneid: 409
Background:
PubMed ID:http://aac.asm.org/content/53/5/1772.abstract
