Product Name: Apaf-1 Antibody [2E10]
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IHC-P, WB
Applications: Apaf1 antibody can be used for detection of Apaf1 by Western blot at 0.25 – 0.5 mg/mL. A 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 0.5 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 130 kDa
Immunogen: A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.
Host Species: Mouse
Purification: Apaf-1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Physical State: Liquid
CAS NO.: 1628316-74-4
Product: MI-136
Buffer: Apaf-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: Apaf-1 monoclonal antibody can be stored at -20˚C, stable for one year.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Apaf-1 Antibody [2E10] : CED4, APAF-1
Accession NO.: AAC51678
Protein Ino: 2330015
Official Symbol: APAF1
Geneid: 317
Background: Apaf-1 Monoclonal Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.
PubMed ID:http://aac.asm.org/content/53/2/557.abstract
